<h2><SPAN name="LESSON_IX" id="LESSON_IX"></SPAN><span class="lght">LESSON IX</span><br/> THE DETERMINATION OF SEX</h2>
<p>The term "The Determination of Sex" is employed in
two general senses in scientific circles.</p>
<p>The first usage is that of the biologist, and it includes
within its scope merely the discovery and understanding
of the <b>causes</b> which determine whether the embryo shall
develop into a male or into a female. In the discussion of
the subject from this standpoint there is but little, if any,
attention given to the question of whether the sex of the
unborn child may be determined by methods under the
control of man. The biologist simply studies the causes
which seem to lead to the production of an individual of
one or the other sex, without regard to whether these
causes, when discovered, may or may not be amendable to
human control.</p>
<p>An authority, speaking of this standpoint concerning
the question referred to, says: "We may discover the
causes of storms or earthquakes, and when our knowledge
of them is sufficiently advanced we may be able to predict
them as successfully as astronomers predict eclipses,
but there is little hope that we shall ever be able to control
them. So it may be with sex; a complete understanding
of the causes which determine it may not necessarily
give us the power of producing one or the other
sex at will, or even of predicting the sex in any given
case. Whether we shall ever be able to influence the
causes of sex-determination cannot as yet be foretold; at
present, biologists are engaged in the less practical, but
immensely interesting, problem, of discovering what those
causes are."</p>
<p>The second usage of the term, includes and embraces<span class="pagenum"><SPAN name="Page_116" id="Page_116">{116}</SPAN></span>
the idea of the voluntary determination or control of the
sex of the future child, by means of certain methods or
certain systems of treatment, etc. Of recent years, science
has been devoting considerable attention to the
question of whether or not man may not be able to produce
any particular sex at will, by means of certain systems
or methods of procedure. Many theories have been
evolved, and many plans and methods have been advocated,
often with the expenditure of much energy and
enthusiasm on the part of the promulgators and their
adherents.</p>
<p>In this lesson there will be briefly presented to you the
general consensus of modern thought on the subject, with
a general outline of the favorite methods and systems
advocated by the several schools of thought concerned
in the investigation.</p>
<p>Professor Doncaster, the well-known authority on the
subject, says: "But little progress has been made in the
direction of predicting the sex of any child, and, if possible,
even less in artificially influencing the determination
of its sex. When the general principles arrived at are
borne in mind, it must be confessed that the prospects of
our ever attaining this power of control or even of prediction
are not very hopeful, but the possibility of it
cannot be yet regarded as entirely excluded. The general
conclusions arrived at are that sex is determined by
a physiological condition of the embryonic cells, that this
condition is induced, at least in the absence of disturbing
causes, by the presence of a particular sex-chromosome.
[A "chromosome" is a portion of the chromatin, or substance
characteristic of the nucleus of the cell, this
nucleus seemingly controlling the life-processes of the
cell.] But there is evidence, which for the present at
least cannot be neglected, that certain extraneous conditions
acting on the egg or early embryo may perhaps be
able to counteract the effect of sex chromosome.</p>
<p><span class="pagenum"><SPAN name="Page_117" id="Page_117">{117}</SPAN></span></p>
<p>"Quite generally, then, there are two conceivable
methods by which the sex might be artificially influenced
in any particular case; firstly, if means could be found
of ensuring that any particular fertilized ovum received
the required chromosomes; and, secondly, by the discovery
of methods which always effect the ovum or embryo
in such a way as to produce the desired sex. Many suggestions
for applying both methods have been made, some
of which have attained considerable notoriety, but hitherto
none of them has stood the test of practical experience.
In the case of the higher animals, especially of
the mammals, in which the embryo develops in the maternal
uterus until long after the sex is irrevocably decided,
it is obviously difficult to apply methods which
might influence the sex after fertilization, even if it were
certainly known that such methods were ever really effective.</p>
<p>"Apart from the few experiments like those of Hertwig
on rearing tadpoles at different temperatures, there
have been a very few cases in which there is even a suggestion
that the sex of the fertilized egg can be modified
by environment, and the belief that this is possible has
been entirely abandoned by many of the leading investigators
of the subject. It is probable, therefore, that if it
will ever be possible to predict or determine artificially
the sex of a particular child, the means will have to be
sought in some method of influencing the output of germ-cells
in such a way that one kind is produced rather than
the other. It is in this way that Heape and others interpret
the results of their investigations; they find that
certain conditions affect the sex-ratio of cells, and they
explain the result by assuming that <b>under some circumstances
male-determining ova are produced in excess, and
under other circumstances, female-determining</b>."</p>
<p>Professor Rumley Dawson holds to the opinion that<span class="pagenum"><SPAN name="Page_118" id="Page_118">{118}</SPAN></span>
the male-determining and female-determining ova are
discharged alternately from the ovaries. In woman one
ovum is usually discharged each month, and it is maintained
that on one month the ovum is male-determining,
and in the next, female-determining. It is obvious that
exceptions must occur, for boy and girl twins are quite
common, but if the cases which support the hypothesis
are taken by themselves, and the exceptions explained
away, it is possible to make out a strong case in favor of
this theory. Some authorities hold that the right ovary
produces male-determining ova, and the left ovary
female-determining, and that the two ovaries discharge
an ovum alternately, but an impartial examination of the
evidence for this belief shows that it rests on very slender
foundations. Experiments on the lower animals have
shown that after the complete removal of one ovary the
female may produce young of both sexes. Women, also,
have produced children of a particular sex after the corresponding
ovary has been removed, and it is hardly possible
to believe that the removal in all these cases was
incomplete. On the whole it must be concluded that the
theory is insufficiently supported by the evidence.</p>
<p>Another widely promulgated and vigorously supported
theory is that which holds that the sex of the future
child may be determined by specific nutrition of the
mother before conception, and in some cases after conception.
Schenk's theory, advanced about 1900, attracted
much attention at the time. He based his method on the
observation that a number of women whose children were
all girls all excreted sugar in their urine, such as happens
in the case of persons affected with diabetes. From this
he suspected that the physiological condition which leads
to the excretion of sugar was inimical to the development
of male-determining ova, and that males could be produced
by its prevention. He therefore recommended<span class="pagenum"><SPAN name="Page_119" id="Page_119">{119}</SPAN></span>
that those who desire a male child should undergo treatment
similar to that prescribed for diabetes for two or
three months before conception, and held that a boy
would be produced by these methods. Although this
method has had considerable vogue, it cannot be held to
have been established on a scientific basis.</p>
<p>Doncaster says "The general conclusion with regard
to man must therefore be that if sex is determined solely
by the spermatozoon there is no hope either of influencing
or predicting it in special cases. On the other hand,
there is considerable evidence that the ovum has some
share in the effect, and if this is so, before any practical
results are reached it will be necessary to discover which
of two conceivable causes of sex-determination is the
true one. It is possible that there are two kinds of ova,
as well as two kinds of spermatozoa, and that there is a
selective fertilization of such a kind that one kind of
spermatozoon only fertilizes one kind of ovum, the second
kind of spermatozoon the second kind of ovum. If this
should prove to be the case, it is possible that means might
be found of influencing or predicting that kind of ovum
which is discharged under any set of conditions. Secondly,
it is possible that the ova are potentially all alike,
but that their physiological condition may under some
circumstances be so altered that the sex is determined
independently of the spermatozoon. * * * It is
hardly possible to avoid the conclusion that the sex of
the offspring may be influenced, at least under certain
circumstances, by the mother. The search for means of
influencing the sex of the offspring through the mother
is not of necessity doomed to failure. No results of a
really positive kind have been obtained hitherto, and
some of the facts point so clearly to sex-determination by
the male germ-cell alone in man and other animals that
many investigators have concluded that the quest is hopeless;
<span class="pagenum"><SPAN name="Page_120" id="Page_120">{120}</SPAN></span>but until an adequate explanation has been given of
certain phenomena discovered in the investigation of the
subject, it seems more reasonable to maintain an open
mind, and to regard the control of sex in man as an
achievement not entirely impossible of realization."</p>
<p>Another writer on the subject has said: "Every individual
among the higher animals, whether male or female,
begins as an impregnated ovum in the mother's
body. Any such ovum contains elements of constitution
from both of its parents. In the earliest existence of this
impregnated ovum, there is a season of sexual indifference,
or indecision, in which the embryo is both male and
female, having the characteristic rudiments of each sex,
only indifferently manifested. In this stage, the embryo
is susceptible of being influenced by external conditions
to develop more strongly in the one or the other direction
and thus become distinctly and permanently male or
female. It is evident that this is the season in the development
of the individual in which influencing conditions
and causes must operate in deciding its sex, although it is
possible in some of the lower animals to alter the tendency
of sex in the embryo from one sex to the other,
even after it has been quite definitely determined. It is
well established, in fact, that differences do not come from
a difference in the ova themselves; that is, there is not
one kind of ova from the female which becomes female,
while other ova become male, for it is possible to alter
the tendency toward the one sex or the other after the
ovum has been fertilized and the embryo has begun its
career of development. This possible change in sex tendency
in the embryo also proves that sex is not decided
by a difference in the spermatozoa; that is some of the
sperm cells from the father are not male, while others are
female, in their constitution.</p>
<p>"It is incorrect to suppose, as has been held by some<span class="pagenum"><SPAN name="Page_121" id="Page_121">{121}</SPAN></span>
theorists, that one testicle give rise to male spermatozoa
and the other to female spermatozoa, for both male and
female offspring have been produced from the same male
parent after one testicle or the other has been removed.
The same is true in cases in which either ovary has been
removed from the mother; that is, male and female offspring
are produced from mothers in whom either ovary
has been removed. In like manner, the sex of offspring
is shown not to be materially affected by the comparative
vigor of the parents; thus, a stronger father than mother
does not necessarily produce one sex to the exclusion of
the other. These negative decisions are important because
they simplify the solution of the problem of sex-determination,
by excluding, more or less fully, various
causes which have been supposed to operate quite forcibly
in deciding the sex of offspring. Some of the more positive
agencies that enter into the determination of sex are
found (1) in the influence of nutrition upon the embryo
during its indifferent stage of sexual development, and
(2) in the constitution and general condition of the
mother before and during the early stages of pregnancy.
These two factors appear to enter more fully than any
others in the decision of the sex in offspring, and deserve
the greatest consideration. The influence of food in supplying
the embryo with nourishment for its development
is, perhaps, the most potent of these determining causes."</p>
<p>Investigators along the line of theory indicated in the
above last quotation, i. e., the theory of sex determination
by means of nourishment of the mother and embryo, have
presented a volume of reports which demand respectful
consideration. The general report may be said to be the
discovery that <b>abundant nourishment during the period
of sexual neutrality tends to produce females; while lack
of abundant nutrition during such period tends to produce
males</b>.</p>
<p><span class="pagenum"><SPAN name="Page_122" id="Page_122">{122}</SPAN></span></p>
<p>These experiments, of course, have been chiefly performed
upon the lower animals. The frog has been a
favorite subject of such experiments—the tadpole stage
being the one selected, because in that stage there exists
a lack of sex, the stage being one of sex neutrality. Professor
Yung's celebrated experiments will illustrate this
class of experiments. Here were chosen 300 tadpoles,
which when left to themselves manifested a ratio of 57
prospective females to 43 prospective males. These were
divided into three classes of 100 tadpoles each. Each
class was then fed upon one of several kinds of nutritious
diet in order to ascertain the change in sex-tendency due
to such food. The first set, with an original ratio of
femaleness of 54 to 46, were fed abundantly on beef, and
the ratio of femaleness was changed to 78 to 22. The
second class, with a ratio of femaleness of 61 to 39, were
fed on fish (specially nourishing to frogs), and the ratio
changed to 81 to 19. The third class, with a ratio of 56
to 44, were fed upon a still more nutritious diet (i. e., that
of frogs' flesh), and the ratio was raised to 92 to 8. In
short, the experiments showed that the increase of nourishment
in diet changed every two out of three male-tendency
tadpoles into females. The experiment was
held to prove that a rich diet, affording nourishment,
during the period of sexual neutrality in the embryo,
tended to develop femaleness.</p>
<p>The advocates of this theory also point to the instance
of the bees. With the bees, the larva of ordinary worker-bees
are fed ordinary food, and do not develop sex; while
the larva which is intended to produce the queen-bee is
fed specially nutritious "royal food," and consequently
develops larger size and full female sex powers. If the
queen is killed, or dies, the hive of bees proceeds to produce
a new queen by means of feeding a selected larva
with the "royal food" and thus developing full femaleness
<span class="pagenum"><SPAN name="Page_123" id="Page_123">{123}</SPAN></span>in it. It is said by some authorities that in cases in
which some other of the larva accidently receive, through
mistake, crumbs of the "royal food," they, too, grow to
an extraordinary size, and develop fertility. This fact is
held by the advocates of the nutrition theory to go toward
establishing the fact that abundant nourishment of the
embryo, during the neutral stage, tends to produce
femaleness in it. They also claim that caterpillars which
are very poorly nourished before entering into the chrysalis
stage usually develop into male butterflies, while
those highly nourished in the said stage tend to become
females. Experiments on sheep have shown that when
the ewes are particularly well nourished the offspring will
show a large proportion of females.</p>
<p>A writer, favoring the theory in question, says: "In
general, it is reasonable to infer that the higher sexual
organization which constitutes the female is to be
attained in the greatest number of cases by embryos
which have superior vital conditions during the formative
period. Among human beings, some facts of general observation
become significant in the light of the foregoing
inferences. After epidemics, after wars, after seasons of
privation and distress, the tendency is toward a majority
of male births. On the other hand, abundant crops, low
prices, peace, contentment and prosperity tend to increase
the number of females born. Mothers in prosperous
families usually have more girls; mothers in families
of distress have more boys. Large, well-fed, fully developed,
healthy women, who are of contented and passive
disposition, generally become mothers of families abounding
in girls; while mothers who are small or spare of
flesh, who are poorly fed, restless, unhappy, overworked,
exhausted by frequent childbearing, or who are reduced
by other causes which waste their vital energies, usually
give birth to a greater number of boys. As a general<span class="pagenum"><SPAN name="Page_124" id="Page_124">{124}</SPAN></span>
proposition, the facts and inferences tend to establish
the truth of the doctrine with women, that, the more
favorable the vital conditions of the mother during the
period in which the sex of her offspring is being determined,
the greater the ratio of females she will bear; the
less favorable her vital conditions at such times, the
greater will be her tendency to bear males. That many
apparent exceptions occur does not disprove the general
tendency here maintained. Moreover, it is impossible to
know in all cases what were the conditions of the mother's
organism at the time in which her child was in its
delicate balance between predominant femaleness and
maleness; else many cases which seemingly disprove the
proposition would be found to be forcible illustrations of
its truth. Still further, it is probable that other causes
besides those here mentioned act with greater or less
effect in determining the sex of offspring."</p>
<p>Based upon this general theory of the relation of
nutrition to sex-determination, many methods and systems
have been devised by as many authorities, and have
been followed and promulgated by as many schools.
Without going into the almost endless detail which would
be necessitated by a synopsis of these various methods
and systems, it may be said that they all consist of plans
having for their object the decrease of nutrition of the
woman in cases in which male children are desired, and
the increase of nutrition in cases in which female children
are sought for. This increase or decrease in nutrition
is enforced for a reasonable period before the time
selected for the conception of the child, and also for a
reasonable period after the time of conception. The decrease
in nutrition does not consist of "starvation," but
rather of a "training diet" similar to that followed by
athletics, and from which dietary all rich foods, sweets,
etc., are absent. In fact, the average dietary advocated<span class="pagenum"><SPAN name="Page_125" id="Page_125">{125}</SPAN></span>
by the "Eat and Grow Thin" writers would seem to be
almost identical with that of the "male offspring" theorists.</p>
<p>Many persons who have followed the methods and
systems based on the nutrition theory above mentioned
claim to have been more or less successful in the production
of the particular sex desired, but many exceptions to
the rule are noted, and some writers on the subject are
disposed to regard the reported successes as mere coincidences,
and claim that the failures are seldom reported
while the successes are widely heralded. The present
writer presents the claims of this school to the attention
of his readers, but without personally positively endorsing
the idea. He is of the opinion that the data obtainable
is not as yet sufficient to justify the strong claims
made for the theory in some quarters; but, at the same
time, he does not hesitate to say that there are many
points of interest brought out in the presentation of the
theory, and that many thoughtful persons seem to accept
the same as reasonably well established and logical.</p>
<p>Another theory which has been heard of frequently of
late years is that in which it is held that the ova are
expelled in alternating sex, each month. Thus, if a male
ovum is expelled in January, the February ovum will be
a female one, according to this theory. Under this theory
if the date of conception of a child be ascertained, and
the sex of the child noted at its birth, it is a simple
matter to count forward from the menstrual period following
which the child was conceived, and thus determine
whether the ovum of any succeeding period is male or
female. It should be noted, however, that the periods
are regulated by the lunar months, and not the calendar
months. The fact that twins of different sexes are sometimes
born would seem to disturb this theory—but not
more than any other theory of sex-determination voluntarily
<span class="pagenum"><SPAN name="Page_126" id="Page_126">{126}</SPAN></span>produced, for that matter. The several schools
explain this apparent discrepancy by the familiar saying
that "exceptions prove the rule."</p>
<p>Another theory of sex-determination is that which
holds that when conception occurs within a few days
after the last day of menstruation, the child will be a girl;
and that when conception occurs at a later period, the
child will be a boy. Methods and systems based upon
this theory are also reported as being reasonably successful
in producing satisfactory results. But, inasmuch as
there appears to be a great difference in individual women
in this respect (even according to the claims of this
school of sex-determination), it would seem that it would
be difficult to proceed with certainty in the matter in
most cases. One of the writers advocating this method,
says: "Conception within five days after the end of the
menstrual period is almost certain to produce a girl child;
within five days to ten days, it may be either a boy or a
girl; from ten to fifteen days, it is almost sure to be a
boy; from eighteen to twenty-five days is the period of
probable sterility, in which conception is extremely unlikely
to occur."</p>
<p>In conclusion, it may be said that Nature undoubtedly
has certain rules of sex-determination which govern in
these cases; and that it is possible if not indeed probable
that these rules may some day be discovered by man, and
turned to account; but that it is very doubtful whether
the secret has as yet been solved by the investigators.
The writer may be pardoned for suggesting that, in his
opinion, if the discovery is ever made it will likely be
found to be very simple—so simple that we have probably
overlooked it because it was in too plain sight to attract
our attention. Nature's methods are usually very simple,
when once discovered. She hides her processes from
man by making them simple, it would seem.</p>
<p><span class="pagenum"><SPAN name="Page_127" id="Page_127">{127}</SPAN></span></p>
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